Skip to main content
. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Cancer Res. 2015 Aug 12;75(17):3479–3491. doi: 10.1158/0008-5472.CAN-14-3587

Figure 3.

Figure 3

Effect of cyclophosphamide (CTX) on the number of MRC1+ VEGFA+ TAMs in LLC1 tumors and VEGFA release by TAMs in vitro. A, representative immunostaining for F4/80+, MRC1+, and VEGFA+. VEGF-expressing F4/80− cells (yellow arrows) and F4/80+ TAMs (orange arrows). B, VEGFA colocalization with MRC1 in TAMs. Bar, 50 μm. C, number of MRC1+ VEGFA+ TAMs. D, VEGFA release by CD45+CD11b+Ly6G−F4/80+ TAMs (VEGFA release in medium over 16 hours, standardized by live TAM numbers). *, P < 0.05.