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. Author manuscript; available in PMC: 2017 Aug 24.
Published in final edited form as: J Am Chem Soc. 2016 Aug 16;138(33):10674–10683. doi: 10.1021/jacs.6b06362

Figure 7.

Figure 7

(A) Time courses of E4(4H) and E4(4D) states in α-70Val→Ile and WT and E4(2N2H) in WT 1 atm N2 turnover in H2O during irradiation with 450 nm diode laser at 12 K. Data points in this and following kinetics plots were obtained as described in Materials and Methods; decays of E4(4H) and E4(4D) are fitted as stretched exponential with following parameters: τ = 9.2 min, m = 0.51 for H2O and τ = 83.3 min, m = 0.45 for D2O. EPR conditions: for this and the following kinetics figures are the same as in Figure 4. (B) Time courses of E4(4H)/ E4(4D) recovery during 193 K annealing of irradiated WT and α-70Val→Ile turnovers in H2O/D2O. Time constants obtained from exponential fit: 8.4 min (H2O) and 41.5 min (D2O), KIE ~ 5.