Figure 2. Feeding responses rely on dopamine tone in AgRP-ablated mice.

(A and B) 4-h cumulative caloric intake of AgRP-ablated (red, blue) or control (black, orange) mice fed with a chow (A) or HFHS diet (B), after a concomitant ICV and intra-VTA injection of vehicle or octanoyled ghrelin and [D-lys3-GHRP-6] or vehicle, respectively. (C, D, E and F) 4-h caloric response (C and E) and cumulative caloric intake (D and F) after a fast in AgRP-ablated (red, rose, orange and brown) or control (black, grey, blue and navy blue) mice presented with chow (black, red, grey and rose) or HFHS diet (blue, orange, navy blue and brown) injected IP with NaCl or a selective dopamine reuptake inhibitor (GBR). (G and H) Spontaneous locomotor activity (G) and caloric intake (H) of 6-month-old lean control animals (black, grey) and AgRP-ablated (red, rose) at the onset of the dark period after an injection IP (45 min before dark period) of the selective D₁R agonist SKF 38393 hydrobromide and selective D₂R agonist quinpirole hydrochloride (SKF+ Quin). n=6 minimum in each group. a, b and c differ significantly (P<0.05). Data are expressed as mean ± SEM. (See also Figure S2).