Fig. 3.

CA activity of Pt43233. (A) CA activity of the purified recombinant Pt43233 in the absence (gray) and presence (green) of Zn. (B) CO₂ hydration (Hyd) and HCO₃⁻ dehydration (Dhyd) activities of cell lysates of WT (open bar) and Pt43233:GFP1 mutant (closed bars) grown under 1% CO₂. The result of the t test is indicated (*P < 0.05). (C) Western blot analysis of Pt43233:GFP following immunoprecipitation with anti-GFP antibody. E, eluted fraction; IP, input; N, nonbinding fraction; ni, nonimmune. The protein band corresponding to Pt43233:GFP is represented by the closed arrowhead, and the IgG protein band is represented by the open arrowhead. (D) CA activity of Pt43233:GFP immunoprecipitated with anti-GFP antibody. As a control, WT and Pt43233:GFP1 (G1) cell lysates were immunoprecipitated with anti-GFP IgG and nonimmune rabbit IgG, respectively. The error bar indicates the SD of three separate experiments.