Fig. S2.

(A) N-terminal sequences of Pt43233 and Tp1093. (Upper) N-terminal sequence of a CGHR family protein, Pt43233, in P. tricornutum is shown. (Lower) N-terminal sequence of the T. pseudonana ortholog of Pt43233 is also shown. ER signals predicted by SignalP are underlined. Chloroplast transit sequences are boxed. Hydrophobic amino acid residues are represented by bold red font. The italicized bold K and R indicate positively charged Lys and Arg preceding the hydrophobic domain. Ala in the TTD AXA motif is represented by bold font. The TTD sequences deleted in the experiment presented in Fig. 2 I and J are indicated by horizontal parentheses with residue numbers in the N-terminal Pt43233 sequence. (B) Relief image conversion of a part of the photograph in Fig. 2E. An enhanced image of the membrane and pyrenoid structures displayed the chloroplast envelope area (dashed red line), the boundary between the stroma and the pyrenoid (dashed yellow line), and thylakoid membrane structures between the chloroplast envelope and the pyrenoid, or in the central part of the pyrenoid (green parentheses).