Fig. S5.
Purification of recombinant Pt43233 protein and determination of esterase activity. (A) Purification of recombinant Pt43233 from E. coli. The N-terminal His6-tagged Pt43233 protein was purified by an IMAC column, followed by His6-tag removal by thrombin cleavage. The fractions were separated by SDS/PAGE and visualized by Coomassie Brilliant Blue (CBB) staining. (B) Time course of esterase activity detected in the absence (open symbols) and presence (closed symbols) of Pt43233. The circles, squares, and triangles represent data from three independent measurements; the arrow indicates the addition point of the substrate, p-nitrophenyl (PNP) acetate.