Table S1.
Protein | Zinc content | CA activity | Esterase activity, nmol⋅min−1⋅mg−1 | |
CO2 hydration | HCO3− dehydration | |||
Pt43233 | 1.43 ± 0.40* | 30.9 ± 0.8† | 42.2 ± 0.8† | 773.9 ± 88.1† |
BH Cytc | 0.10 ± 0.04† | N.D. | N.D. | N.D. |
BSA | N.D. | 0.03 ± 0.03† | 0.04 ± 0.05† | −0.8 ± 59.4† |
Zinc content (mol⋅mol−1 protein), CA activity (WAU⋅mg−1 protein), and esterase activity (nmol⋅min−1⋅mg−1 protein) are listed for purified recombinant Pt43233 and two negative control proteins: bovine heart cytochrome c (BH Cytc) and BSA. N.D., not determined.
Values are the mean ± SD of the four separate measurements.
Values are the mean ± SD of the three separate measurements.