Fig. 2.
Systemic PAMAM-G3 treatment reduces glomerular immune complex pathology and C3c deposits in MRLlpr mice. (A) Representative H&E histology of paraffin-embedded renal sections from PBS and PAMAM-G3 MRLlpr treated mice as well as wild-type control (untreated) mice. Data show representative sections from 18 mice per group. (Scale bars: 50 µm.) (B) Glomerulonephritis evaluation of kidneys after PBS/PAMAM-G3 treatment. Data are from three independent experiments. Each circle represents one mouse. n = 18. *P < 0.05. (C) Frozen renal sections from PBS and PAMAM-G3 MRLlpr treated mice as well as wild-type control (untreated) mice were stained for complement factor C3c. Data show representative sections from 15 mice per group. Magnification, 20×. (D) Fluorescence intensity of the C3c staining was measured using ImageJ. Data are composites of three independent experiments. Each circle represents one mouse. n = 15. ***P < 0.001.