Fig. S2.

NASs block TLR activation by nucleic acid agonists but not LPS in DCs isolated from NZBW F₁ animals similarly to wild-type mice. (A and B) NASs block IL-6 and TNFα cytokine production during CpG but not LPS stimulation in both wild-type and lupus-prone (NZBW F₁) mice. Bone marrow-derived dendritic cells (DCs) were cultured as previously described. DCs were then cultured in the presence of LPS and CpG as well as 20 µg/mL of each NAS (HDMBr, CDP, and PAMAM-G3). IL-6 and TNFα cytokine production was assessed 18 h later using ELISA. Data are representative of three independent experiments. n = 9 mice per group. **P < 0.01.