Fig. 1.

p53 is monomethylated at K370 and K382 in teratocarcinoma cells. (A) Western blot analysis and quantification of protein levels in NTera2 and other cell lines with wild-type p53. For each blot, protein amount was set to 1 in NTera2 cells, and relative protein levels were denoted in other cell lines for comparison. (B) Nuclear-cytosolic fractionation and examination of p53 methylation in NTera2 cells by p53 immunoprecipitation, followed by p53 K370me1 and p53 K382me1 Western blot analysis. (C) Nuclear-cytosolic fractionation and examination of p53 methylation in Tera1 cells by p53 immunoprecipitation followed by p53 K370me1 and p53 K382me1 Western blot analysis. *Nonspecific band.