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. 2016 Aug 17;113(35):9822–9827. doi: 10.1073/pnas.1610387113

Fig. 3.

Fig. 3.

SMYD2 or PR-Set7 knockdown activates p53 transcriptional activity and promotes a differentiation feature of NTera2 cells. (A) Reverse transcription followed by qPCR (RT-qPCR) analysis of mRNA levels in NTera2 cells with control (Ctrl) or SMYD2 knockdown mediated by shRNA. No. 1 and no. 2 indicate two different shRNA constructs. (B) Western blot analysis of protein levels in NTera2 cells with control or SMYD2 knockdown mediated by shRNA. (C) RT-qPCR analysis of mRNA levels in NTera2 cells with control or PR-Set7 knockdown mediated by shRNA. (D) Western blot analysis of protein levels in NTera2 cells with control or PR-Set7 knockdown mediated by shRNA. (E) RT-qPCR analysis of mRNA levels in NTera2 cells with control sgRNA or sgRNA targeting PR-Set7 for gene “knockdown” at a cell population level. (F) Western blot analysis of protein levels in NTera2 cells with control sgRNA or sgRNA targeting PR-Set7 for gene “knockdown” at a cell population level. (Error bars represent mean ± SEM; n = 3; two-tailed Student’s t test: *P < 0.05; **P < 0.02; ***P < 0.01).