Table S1.

Panning data for the selection of scFv antibodies against hERG PAS domain

Panning cycle no.	Solution-phase selection				Solid-phase selection: PAS
	GSTPAS_1	GST_1	GSTPAS_2	GST_2
1
Eluted phage (% of input)	0.333000		0.181000		0.000034
Enrichment*	n.d.		n.d.		1
2
Eluted phage (% of input)	0.000094		0.000128		0.004400
Enrichment*	1		1		129
3
Eluted phage (% of input)	0.028		0.044		0.115
Enrichment*	297.9		343.8		3,382
4
Eluted phage (% of input)	0.19300	0.00083	0.35600	0.00131	0.09600
Enrichment*	2,053	0.03	2,781	0.03	2,823

GSTPAS_1 and GSTPAS_2 were purified as fusion proteins and coupled to magnetic beads for screening. GST_1 and GST_2 were purified as control. PAS protein was purified and coated onto Nunc Maxisorp plates for screening. n.d., not determined.

^*Enrichment was calculated as the ratio between the eluted phage in a certain cycle and the eluted phage in the first cycle.