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. 2016 Aug 15;113(35):9786–9791. doi: 10.1073/pnas.1610103113

Table S1.

Parameters for each round of in vitro evolution

Round Method Nt added Time (h) Primer Template Splint Mut
1 gel 20 24 SP1 T-R0 Spl1
2 gel 20 24 SP1 T-R0 Spl1
3 gel 20 24 SP1 T-R0 Spl1
4 gel 20 24 SP1 T-R0 Spl1 +
5 apt 12 4 SP2 T-B12 Spl2
6 apt 12 4 SP2 T-B12 Spl2
7 apt 12 4 SP2 T-B12 Spl2
8 gel 18 24 SP3 T-B12 Spl2
9 gel 18 24 SP3 T-B12 Spl2
10 gel 18 24 SP3 T-B12 Spl2
11 gel 18 24 SP3 T-B12 Spl2
12 gel 18 24 SP3 T-B12 Spl2 +
13 gel 30 2 SP4 T-GTP Spl3
14 gel 30 2 SP4 T-GTP Spl3
15 gel 30 2 SP4 T-GTP Spl3
16 gel 30 2 SP4 T-GTP Spl3
17 gel+apt 30 2 SP4 T-GTP Spl3 +
18 gel 32 6 SP1 T-R12 Spl1
19 gel 32 1 SP1 T-R12 Spl1
20 gel 32 0.25 SP1 T-R12 Spl1
21 gel 40 0.25 SP1 T-R20 Spl1
22 gel+apt 30 0.25 SP4 T-GTP Spl3 +
23 gel+apt 30 0.25 SP4 T-GTP Spl3
24 gel+apt 30 0.25 SP4 T-GTP Spl3

The selection method involved gel-shift (gel) and/or aptamer capture (apt), following polymerase extension of 12–40 nt for 0.25–24 h. See Table S4 for sequences of primers, templates, and splints. Mutagenic PCR was performed after certain rounds, as indicated by +.