Table S2.
Polymerase | Expected | Observed (%) | |||||
A | C | G | U | Del | Ins | ||
WT | A | 91.3 | 1.0 | 1.4 | 0.1 | 6.2 | 0.0 |
C | 0.0 | 99.1 | 0.1 | 0.8 | 0.0 | 0.0 | |
G | 0.1 | 0.7 | 99.0 | 0.2 | 0.0 | 0.0 | |
U | 0.7 | 0.2 | 1.6 | 97.4 | 0.1 | 0.1 | |
24-3 | A | 81.1 | 0.4 | 7.4 | 0.4 | 10.7 | 0.0 |
C | 0.2 | 93.3 | 0.2 | 6.0 | 0.3 | 0.0 | |
G | 0.3 | 0.6 | 98.2 | 0.2 | 0.6 | 0.0 | |
U | 0.4 | 0.4 | 1.5 | 96.5 | 1.3 | 0.1 |
Operating on the favorable template 3′-ACGCUUCGCAC-5′, the average fidelities of WT and 24-3 were 96.6% and 92.0%, respectively, calculated as the geometric mean of the fidelities for each templating base, including deletions. Excluding wobble mutations, the fidelities were 97.2% and 95.5%, respectively. Bold values indicate correct nt incorporation.