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Mutations during riboPCR
| Input | Output (of 27) | Consensus | ||||
| A | C | G | U | Del | ||
| A1 | 26 | 0 | 1 | 0 | 0 | A |
| G2 | 1 | 0 | 26 | 0 | 0 | G |
| A3 | 15 | 1 | 5 | 2 | 4 | A |
| G4 | 0 | 0 | 20 | 0 | 7 | G |
Observed mutations in the central AGAG region of a 24-nt template after 100-fold amplification by riboPCR. The consensus from 27 sequenced clones is shown at the right. Bold values indicate correct nt incorporation.
