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CsrAcrw proteins bind to RNA in vitro. (A–C) RNA EMSA, using WT hag 5′UTR RNA and the indicated amounts of CsrA–His6 (A), CsrAN55D–His6 (B), or CsrAI14M–His6 (C) and FliW protein. “F” indicates unbound RNA, “C” indicates CsrA–RNA complex, and “W” indicates FliW–RNA complex. ”I” indicates a band with slightly increased mobility that might suggest a release intermediate.
