Fig. S1.

CsrA^lof alleles in the RNA binding pocket are stable. (A) Each residue mutated in a CsrA lof allele, originally isolated as suppressors of fliW (sow), is highlighted on the structure of the homologous protein RsmA from Pseudomonas aeruginosa (15, 38). The RNA binding surface is indicated by a dotted line. (B) Western blot analysis of B. subtilis cell lysates of WT (3610), and 10 unique missense CsrA^lof mutants (DS6529, DS6604, DS6608, DS6632, DS6633, DS6634, DS8579, DS8580, DS8584, DS8592) separately probed with anti-CsrA, and anti-SigA primary antibodies.