Table S7.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source |
| Strains | ||
| BL21-Gold(DE3) | E. coli B, F– ompT hsdS(rB– mB–) dcm+ gal λ(DE3) endA Hte, TetR | Stratagene |
| X90 | F´ lacIq lac´ pro´/ara ∆(lac-pro) nal1 argE(amb) rifr thi-1, RifR | (39) |
| CH1944 | X90 (DE3) ∆rna, RifR | (38) |
| CH2016 | X90 (DE3) ∆rna ∆slyD::kan, RifR KanR | (38) |
| CH7157 | X90 ∆clpX ∆clpA::kan, RifR KanR | (31) |
| CH8164 | X90 ∆cysK::kan, RifR KanR | (8) |
| CH8602 | X90 ∆cysK, RifR | (8) |
| CH8804 | X90 (DE3) ∆rna ∆slyD ∆cysK::kan, RifR KanR | This study |
| CH9648 | X90 (DE3) ∆rna ∆slyD ∆tufB tufA-his6-kan, RifR KanR | This study |
| CH10013 | Spontaneous rifampicin-resistant derivative of E. coli JCM158, RifR | (5) |
| CH10801 | CH10013 ∆cysK, RifR | This study |
| CH11002 | CH8804 pCH11014 pCH13129, RifR KanR TetR AmpR | This study |
| CH12810 | CH8804 pCH8649 pET21, RifR KanR TetR AmpR | This study |
| CH12811 | CH8804 pCH8649 pCH8501, RifR KanR TetR AmpR | This study |
| CH12812 | CH8804 pCH12817 pET21, RifR KanR TetR AmpR | This study |
| CH12813 | CH8804 pCH12817 pCH11027, RifR KanR TetR AmpR | This study |
| CH13101 | CH8804 pCH11014 pET21, RifR KanR TetR AmpR | This study |
| CH13102 | CH8804 pCH11014 pCH12515, RifR KanR TetR AmpR | This study |
| Plasmids | ||
| pTrc99a | IPTG-inducible expression plasmid, AmpR | GE Healthcare |
| pCH450 | pACYC184 derivative containing E. coli araC and the l-arabinose–inducible Para promoter, TetR | (38) |
| pET21 | T7 RNA polymerase-based overexpression vector, AmpR | (3) |
| pCH6190 | pET21::cdiA-CT/cdiIEC536, overproduces CdiA-CTEC536 and CdiIEC536-His6, AmpR | (3) |
| pCH6316 | pTrc99A::cdiA-CT/cdiIEC536(DAS), AmpR | (30) |
| pCH6938 | pET21::cdiA-CT(H178A)/cdiIEC536, overproduces CdiA-CT(H178A)EC536 and CdiIEC536-His6, AmpR | (8) |
| pCH7086 | pCH450::cdiA-CT(H178A)EC536, TetR | (8) |
| pCH7171 | pCH450::cdiA-CT/cdiIEC536(DAS), TetR | This study |
| pCH8215 | pET21::cysK(S2G), overproduces CysK-His6, AmpR | (8) |
| pCH8501 | pET21::cdiA-CT(H178A)EC536, AmpR | This study |
| pCH8649 | pCH450::cysK(S2G)-his6, TetR | (8) |
| pCH9320 | pCH450::cdiA-CTEC536, TetR | (8) |
| pCH9622 | pKAN-tufA(his6), AmpR KanR | This study |
| pCH10978 | pET21::cdiA-CT(D155A)/cdiIEC536, AmpR | This study |
| pCH10980 | pET21::cdiA-CT(W176A)/cdiIEC536, AmpR | This study |
| pCH10982 | pET21::cdiA-CT(E181A)/cdiIEC536, AmpR | This study |
| pCH11022 | pET21::cdiA-CT(T185I)/cdiIEC536, overproduces CdiA-CT(T185I) and CdiIEC536-His6, AmpR | This study |
| pCH11014 | pCH450::cysK(S2G), expresses untagged CysK, TetR | This study |
| pCH11016 | pCH450::cdiA-CT(D155A)EC536, TetR | This study |
| pCH11017 | pCH450::cdiA-CT(W176A)EC536, TetR | This study |
| pCH11019 | pCH450::cdiA-CT(E181A)EC536, TetR | This study |
| pCH11020 | pCH450::cdiA-CT(T185A)EC536, TetR | This study |
| pCH11022 | pET21P::cdiA-CT(T185A)/cdiIEC536, AmpR | This study |
| pCH11027 | pET21::cysK(S2G), overproduces untagged CysK, AmpR | This study |
| pCH12305 | pET21::tox/immRlac, overproduces Ntox28Rlac and ImmRlac-His6, AmpR | This study |
| pCH12371 | pCH450::cdiA-CT(N149D)EC536, TetR | This study |
| pCH12372 | pCH450::cdiA-CT(K152A)EC536, TetR | This study |
| pCH12373 | pCH450::cdiA-CT(R187A)EC536, TetR | This study |
| pCH12334 | pCH450::tox/immRlac(DAS), TetR | This study |
| pCH12515 | pET21::his6-cdiA-CT(H178A)/cdiIEC536; overproduces His6-CdiA-CT(H178A)EC536 and CdiIEC536, AmpR | This study |
| pCH12817 | pCH450::his6-cdiA-CT(H178A)EC536, TetR | This study |
| pCH13103 | pET21::his6-tox/immRlac, overproduces His6-Ntox28Rlac and ImmRlac, AmpR | This study |
| pCH13129 | pET21::his6-cdiA-CT(H178A)EC536, overproduces His6-CdiA-CTEC536, AmpR | This study |
| pCH13146 | pET21::his6-tox(H114A)/immRlac, overproduces His6-Ntox28(H114A)Rlac and ImmRlac, AmpR | This study |
| pCH13153 | pET21::his6-tox(H114A)Rlac, overproduces His6-Ntox28(H114A)Rlac, AmpR | This study |
AmpR, ampicillin-resistant; CmR, chloramphenicol-resistant; KanR, kanamycin-resistant; RifR, rifampicin-resistant; TetR, tetracycline-resistant.