Fig. 2.

Effects of JAK inhibition on the immunological transcriptome. Immunocytes were sorted from JAKi-treated mice (repeated dosing, twice daily, 1 wk), and mRNAs were profiled on genomewide microarrays. (A) Range and significance of changes: fold change vs. P value (“Volcano”) plots for B cells (Left) and MF (Right) from Tofa-treated mice; all expressed genes are shown. (B) Drug specificity: fold change/fold change plot comparing effects of Tofa and JAK1i (Left) or Tofa and JAK3i (Right) in B cells. (C) Heat map of fold changes in the JAKi-affected gene set in six different splenic cell types, after treatment with different compounds (fold changes for each sample calculated vs. mean of all vehicle-treated samples). Cluster assignment is shown at right.