Table 1.
Overview of mating combinations between different M. canettii and M. tuberculosis strains
| M. canettii Hygr donor strains | Kmr receipient strains | MTBC Hygr donor strains | Kmr receipient strains |
| Mcan-A, | |||
| Mcan-K, | |||
| Mcan-K, | Mtb-H37Rv | Mcan-L, | |
| Mcan-A | Mcan-L, | Mcan-J, | |
| Mtb-H37Rv, | Mtb-79112, | ||
| H37RvΔRD1 | Mtb-Tb36 | ||
| Mcan-A, | |||
| Mcan-K, | |||
| Mcan-I | Mcan-L, | Mtb-79112 | Mtb-H37Rv |
| Mtb-H37Rv, | Mtb-Tb36 | ||
| H37RvΔRD1 | |||
| Mcan-A, | |||
| Mcan-K, | Mtb-Tb36 | Mtb-H37Rv | |
| Mcan-J | Mcan-L, | Mtb-79112 | |
| Mtb-H37Rv, | |||
| H37RvΔRD1 | |||
| Mcan-A, | Mcan-A, | ||
| Mcan-K | Mcan-L, | H37RvΔRD1 | Mcan-K, |
| Mtb-H37Rv, | Mcan-L, | ||
| H37RvΔRD1 | Mtb-H37Rv | ||
| Mcan-A, | |||
| Mcan-L | Mcan-K, | ||
| Mtb-H37Rv, | |||
| H37RvΔRD1, |
Donor strains carrying the pYUB412 or F10 cosmid were used, as well as recipient strains carrying the pMRF1-dsRed replicative plasmid, except for M. canettii J, which contains an integrative pMV306.Kan plasmid-mediated insertion, due to a putative restricted plasmid maintenance, linked to the presence of the eptABCD gene cluster. M. canettii donor strains A, K, L, and I additionally carried spontaneous rifampicin resistance mutations. Only for mating pair M. canettii A and M. canettii L recombinants (bold) were obtained.