Fig. 5.

Constitutive expression of PLATZ1 confers tolerance to low water availability in vegetative tissues. 35S::PLATZ1/Col-0-overexpressing lines display increased tolerance to low water availability under both greenhouse (A and B) and growth chamber (C and D) conditions. Plants were grown under greenhouse conditions for 20 d and then subjected to a low water availability treatment by withholding water irrigation for 15 d; after the drought treatment, plants were evaluated 5 d after being irrigated once to field capacity. (A, Upper) Transgenic and WT plants before the low water availability treatment. (A, Lower) 35S::PLATZ1 and control plants 5 d after the low water availability treatment (flowering stems were cut to show the rosette leaves). (B) Percentage of surviving plants after the low water availability treatment and the recovery irrigation. Values are the means ± SD of three biological replicates (n ≥ 18). (C) Independent pots with Arabidopsis plants grown under growth chamber conditions were subjected to a low water availability stress in which water was withheld for 15 d and then subjected to a recovery irrigation treatment; images of representative Col-0 and 35S::PLATZ1 plants 5 d after the recovery irrigation are shown (flowering stems were cut to show the rosette leaves). (D) Percentage of surviving plants after the low water availability treatment and the recovery irrigation. Values are means ± SD of three biological replicates (n ≥ 18). Growth chamber conditions were 22 °C with a 16-h photoperiod of 200 μmol⋅m⁻²⋅s⁻¹.