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. 2016 Aug 17;113(35):E5153–E5162. doi: 10.1073/pnas.1602739113

Fig. S6.

Fig. S6.

Differential inactivation of EGF signaling by pnt RNAi. (A) EGF signaling was strongly reduced by optix-Gal4 UAS-pnt RNAiJF02227 at 30 °C. GFP (green), L'sc (blue), and PntP1 (magenta). Expression of L'sc and PntP1 was completely lost, except for a small number of L'sc-positive cells in the lateral region of the optic lobe (arrow). (B–D) The effects of pnt RNAi on eye development were compared under the control of GMR-Gal4 at 25 °C. (B) Control. (C) UAS-pnt RNAiJF02227 caused mild reduction in the eye size. (D) UAS-pnt RNAiHMS01452 caused severe reduction in the eye size. (E) EGF signaling was strongly reduced by optix-Gal4 UAS-pnt RNAiHMS01452 at 25 °C. GFP (green) and L'sc (blue). The proneural wave as visualized by L'sc expression was eliminated. Optic stalk is indicated by an arrow.