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. 2016 Sep 8;57(11):4704–4712. doi: 10.1167/iovs.15-18663

Figure 3.

Figure 3

Oxidized LDL taken up by RPE is targeted to the lysosomes through the CD36 receptor. hf-RPE and ARPE-19 cells grown on laminin-coated coverslips for a week were treated with 10 μg/mL DiI-ox-LDL and then visualized at 15 hours by immunofluorescent staining for LAMP-1 to localize the lysosomes. For function blocking assay, ARPE-19 cells were treated with 10 μg/mL DiI-ox-LDL in the presence of 20 or 40 μg/mL of control IgA or anti-CD36 IgA. At 15 hours posttreatment, there was clear localization of DiI-ox-LDL to LAMP-1-staining lysosomes in (A–C) hf-RPE and (D–F) ARPE-19. Anti-CD36 IgA treatment (40 μg/mL) significantly decreased DiI-ox-LDL uptake in ARPE-19 cells (H). (I) Quantification of the number of DiI-ox-LDL particles present per field of view.

** P < 0.01. Arrows in (C) and (F) show the location of DiI-ox-LDL within LAMP-1–positive lysosomes. Scale bars: 10 μm (A–F) and 50 μm (G, H).