TABLE 4.
MTR rs1805087 genotype alters plasma choline metabolite partitioning and flux
| MTR rs1805087 group | 480 mg/d choline |
930 mg/d choline |
||||
|---|---|---|---|---|---|---|
| WT | Variant | P | WT | Variant | P | |
| Betaine-d9/PC-d9 | ||||||
| RS × gene interaction; P = 0.06 | ||||||
| Lactating | 0.81 ± 0.03 | 0.80 ± 0.04 | >0.99 | 0.96 ± 0.03 | 0.95 ± 0.04 | >0.99 |
| NP | 0.74 ± 0.03 | 0.86 ± 0.04 | 0.07 | 0.90 ± 0.03 | 1.01 ± 0.04 | 0.07 |
| Pregnant | 0.65 ± 0.03 | 0.63 ± 0.04 | >0.99 | 0.81 ± 0.03 | 0.78 ± 0.04 | >0.99 |
| Choline → betaine | ||||||
| 3-Way interaction; P = 0.008** | ||||||
| Lactating | 41 ± 5 | 40 ± 7 | >0.99 | 46 ± 5 | 48 ± 7 | >0.99 |
| NP | 33 ± 6 | 23 ± 7 | >0.99 | 50 ± 5 | 94 ± 9 | <0.001** |
| Pregnant | 12 ± 5 | 16 ± 9 | >0.99 | 19 ± 5 | 18 ± 7 | >0.99 |
| Betaine → methionine | ||||||
| Cho × gene interaction; P = 0.001*** | ||||||
| Lactating | 1.31 ± 0.09 | 1.54 ± 0.11 | 0.1 | 1.44 ± 0.09 | 1.18 ± 0.11 | 0.05* |
| NP | 1.42 ± 0.10 | 1.65 ± 0.11 | 0.1 | 1.76 ± 0.09 | 1.49 ± 0.11 | 0.05* |
| Pregnant | 1.79 ± 0.08 | 2.02 ± 0.11 | 0.1 | 2.29 ± 0.09 | 2.03 ± 0.10 | 0.05* |
Values are least-squares means ± se. Betaine-d9/PC-d9 values are ratios, choline → betaine values are in micromolar betaine/study period, and betaine → methionine values are in micromolar methionine/study period. Cho, choline intake level. P values represent the highest order interaction or main effect; symbols denote significance at the indicated α-cutoff. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.