Figure 6.

Knockdown of BmHRG-1 by hsiRNA soaking. A) B. malayi females were cultured in RPMI-1640 medium containing increasing concentrations of BmHRG-1 hsiRNA (0, 1, or 5 μM). Medium was changed every 12 h, at which point mf output for each treatment group was determined. Each hsiRNA treatment group mf count is normalized based on the 0-h timepoint. B) B. malayi females (6 worms/treatment group) were pretreated with or without 1 μM BmHRG-1 hsiRNA for 24 h (d −1 to 0) before the addition of 20 μM GaPPIX (d 0). Motility was assessed as described in Materials and Methods on a scale from 0 (nonmotile) to 4 (highly motile). Each data point represents motility from a single experiment where worms were scored as a group (6 adult worms/treatment group). At d 3, 20 μM GaPPIX was added to the RNAi-only treatment group (red arrow) to determine if longer pretreatment with BmHRG-1 hsiRNA would provide significant protection from GaPPIX cytotoxicity.