TABLE 4.

ATP levels in controls and premutation PBMCs

Pathway	Control (n = 11)	FXTAS-free (n = 21)	FXTAS-affected (n = 9)	P
Oxphos	15 ± 4 (67%)	5.2 ± 0.6 (38%)	2.9 ± 0.8 (27%)	0.019
Glycolysis	7.3 ± 0.9 (33%)	8.4 ± 0.4 (62%)	8 ± 2 (73%)	0.390
Total ATP	22.3 ± 1.2	13.6 ± 0.2	10.9 ± 0.2	0.0001

ATP levels from oxphos were calculated [as nanomoles ATP × (glucose × 10⁶ cells)⁻¹] (means ± sem) (%)] by the oligomycin-sensitive oxygen uptake of glucose-supplemented intact cells and normalized by glucose consumed per million cells. ATP from glycolysis was calculated from the lactate-to-glucose ratio obtained by metabolomics analysis and normalized as above. In parentheses, the percentage of total ATP produced by each pathway. P values were computed by Student’s t test for comparisons between FXTAS-free and -affected individuals.