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. 2016 Jul 11;30(10):3515–3526. doi: 10.1096/fj.201500040

Figure 1.

Figure 1.

HKa selectively stimulates p56/Lck expression and phosphorylation in endothelial cells. Proliferating HUVECs were cultured in absence or presence of 15 nM HKa for indicated times, and cell extracts were prepared and subjected to immunoblotting. A) HKa specifically induces expression of p56/Lck but does not affect other SFK members. B) Concentration-dependent stimulation of p56/Lck expression by HKa. C) Time course of p56/Lck and p56/Lck phosphorylation on Y394 (associated with active form of p56/Lck) after exposure of endothelial cells to 15 nM HKa. D) Analysis of p56/Lck Y394 and Y505 phosphorylation after exposure to HKa.