Figure 2.
Open probability for hClC-1 WT, T335N, and WT + T335N hClC-1 channels in high intracellular chloride. A–C) Representative tail current traces elicited from tsA cells transfected with WT (A), T335N (B), and WT+T335N (C) from experiments to determine overall gating (top) and slow gating (bottom) in high intracellular chloride solution. D–F) Apparent Po for overall (D), slow (E), and fast gating (F) plotted as a function of test voltage, for WT, T335N, and WT + T335N channels. Relationships obtained from averaged data were fit with a Boltzmann equation. Solid lines represent the WT currents. Open probability for slow gating was obtained as described in Supplemental Data. Fit parameters are reported in Tables 1 and 2. Each point is mean ± sem from 7–11 cells.