TABLE 1.

Biophysical parameters for hClC-1 WT, T335N, and WT + T335N channels

ClC-1	[Cl−] (mM)	V0.5 Pototal[mV (k)]	Pmintotal	IC (pA/pF)	SSC (pA/pF)	Cells (n)
WT	134	−74 ± 1 (30 ± 1)	0.45 ± 0.04	−72 ± 6.7	50.5 ± 6	15
T335N	134	94 ± 5* (25 ± 2)	0.68 ± 0.06	−13 ± 2.9*	7.6 ± 2.2*	13
WT + T335N	134	−50 ± 3 (51 ± 2)	0.39 ± 0.03	−59 ± 6	31 ± 3.8	18
WT	4	−22.9 ± 1.3 (37 ± 1)	0.23 ± 0.03	—	35 ± 8.9	8
T335N	4	39.1 ± 4.4 (72 ± 5)*	0.45 ± 0.03*	—	5.8 ± 1*	10
WT + T335N	4	30.1 ± 6.7 (74 ± 8)*	0.17 ± 0.03	—	17.9 ± 6.3*	7

Data are means ± sem of the indicated number of cells. V_0.5, half maximal activation potential; k, slope factor; P_min, minimal value of P_o measured at −90 mV; IC, instantaneous current measured at −60 mV; SSC, steady state current measured at +60 mV. *P < 0.05 compared with WT.