Figure 5.

Simulation results in the context of the parameter space of a model involving water and solutes transport (WS model). A small portion of the parameter space is shown in (A). The parameter space shows all the possible steady volumes (in color code) predicted by the WS model. The P_f and P_s values obtained by fitting simulations are superimposed and shown as mean ± SEM. (B) Relationship between the P_f/P_s ratio and pH. Symbols represent the mean ± SEM of the P_f/P_s ratio. Continuous line shows the fitting to an exponential function $\left(P_f/P_s=A·e^{\left(-\left(pH-pH\ast \right)/p{H}^{\prime }\right)}+P_f/P_s^{\ast }\right)$. Fitting parameters are: A = −36.5 ± 1.4; $P_f/P_s^{*}$ = 36.3 ± 1.1; pH^* = 6.6; and pH' = 0.73 ± 0.09; R² > 0.99). (C) Relationship between the steady volume (V_f/V₀) and the P_f/P_s ratio. Symbols represent the mean ± SEM of both the P_f/P_s and the V_f/V₀ ratios obtained with the WS model. Continuous line shows the result of a linear fitting (intercept: 1.00 ± 0.002, slope: 0.0025 ± 0.0001, R² > 0.99). Dash lines in (B,C) represent the 95% CI. In both panels, open circles are data from experiments performed in this work (pH 7.0 and 6.8), and black circles are data taken from previous experiments (Amodeo et al., 2002).