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. 2016 Sep 15;7:1357. doi: 10.3389/fpls.2016.01357

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Copyright © 2016 Cui, Liu, Islam, Li, Farooq, Ruan and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation of interaction between OsCYP2 and OsPEX11 protein using in vitro assay. Recombinant glutathione-S-transferase (GST) and GST-OsCYP2 was used as bait and incubated with 1 μg of prey protein (His-OsPEX11), respectively. After incubation, GST and GST-OsCYP2 were retrieved with glutathione beads, and the pulled-down proteins were detected on Western blots with antibodies to Histidine. GST: pGEX-4T-1; GST-OsCYP2: pGEX-4T-1+OsCYP2; His-PEX11: pET28a+OsPEX11.