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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Cell Rep. 2016 Sep 13;16(11):2838–2845. doi: 10.1016/j.celrep.2016.08.040

Figure 4.

Figure 4

OLIG2 is the Nuclear Gateway for Proneural-Mesenchymal Transition. A. hGSCs (BT145, GB3) were transduced with shNT (non-target control) or shOLIG2. Lysates were collected 48 hours post-transduction, and immunoblotted for the indicated proteins. B-C. Lysates from hGSCs expressing control GFP, WT-Olig2 (V5-tag), or ΔOlig2 (FLAG-tag) were collected and immunoblotted for CD44, GFAP, and ectopic Olig2. Note: blots presented here were reprobed from Figure 3C-D. D. qRT-PCR analysis of hGSCs (BT145, GB16) expressing control GFP or ΔOlig2 for indicated subclass genes. HPRT and 18s were utilized as housekeeping genes. (n ≥ 3). E. Proposed model of OLIG2-mediated regulation of growth factor signaling. See also Figure S4.