Fig 3. Myogenic differentiation of skeletal muscle microtissues.
(A) Nuclei (red) and myogenin (green) labelling of 10x constructs at Day 2 of differentiation. Scale bar: 50 µm. (B) Quantification of myogenin-positive nuclei in 10x constructs at Day 2 of differentiation (n=3). (C) Confocal microscopy images of troponin T (green) and nuclei (red) staining of 10x constructs after 4 days in differentiation medium. Scale bar: 100 µm.