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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Apr 26;474(3):515–521. doi: 10.1016/j.bbrc.2016.04.130

Fig 4. Rho-kinase inhibitor improves myogenic differentiation in FN/G-coated thick constructs.

Fig 4

(A) Effects of the Rho-kinase inhibitor Y27632 (Y27) on myogenic differentiation: observation of the insert periphery after 5 days of differentiation. Scale bar: 100 µm. (B) Confocal microscopy images of troponin T (green) and nuclei (red) staining of C2C12 cell constructs after 5 days in differentiation medium supplemented or not with 5 µM Y27632. Scale bar: 50 µm. (C) Fusion index of 10x FN/G-coated constructs at Day 5 of differentiation (n=2, *p<0.05 compared to all other conditions). (D) Phase contrast microscopy observations of hematoxylin-eosin stained cross-sections in Z of FN/G-coated and non-coated constructs after 4 days of culture in DM. Scale bar: 50 µm. (E) Quantification of the thickness from histological cross-sections after 4 days of culture in DM (n=2, *p<0.05).