Figure 6.

miR-23a induces the proliferation of hematopoietic cells via downregulation of RKIP. A, U937 with stable expression of FLAG-RKIP and empty vector, respectively, were transfected with either miR-23a mimic or unspecific control (Cntrl) as indicated. In addition, parental U937 cells were lentivirally transduced with either RKIP shRNA or control. Cells were seeded and maintained as described in Materials and Methods and viable cells were counted after 5 days. For comparison of the different conditions, respective Cntrl situations were set at a value of 1 and the relative increase of viable cells in the miR-23a/RKIP shRNA conditions was calculated using the ratio viable cells miR-23a/RKIP shRNA to viable cells control. B–D, BrdUrd/7-AAD cell-cycle/proliferation assays were performed in all the settings described above to evaluate the percentage of cells in S-phase (top gate), G₀–G₁-phase (left bottom gate), and G₂–M-phase (right bottom gate), respectively. Black bars, control; white bars, miR-23a/RKIP shRNA. The graphs summarize the results of at least three independent experiments. Data are expressed as mean values ± SD; *, P < 0.05; **, P < 0.01.