Figure 2.

Schematic of the products from the TU confirmation PCR of the sample (A) CTA2 and (B) CTA4. PCR products were separated on 1% agarose and stained with GelRed. Lane M, HyperLadder 1 kb (Bioline, UK). The open arrowed boxes represent ORFs, pointing in the probable direction of transcription. The IS elements and ORF in the DNA segment are shown in blue and green, respectively. The dashed arrow box represents truncated gene. The red arrows represent the binding site of the TU verification primers. The brown arrows indicate the expected PCR products of the TU verification PCR. The black dashed lines indicated the expected size of the amplicons.