Table 2. Molecular characterization of M. bovis isolates from cattle in Midwest Brazil.

Sample	Spoligotype pattern	Spoligotype Cluster	24-MIRU-VNTR profile	MIRU-VNTR cluster	Combined analyses cluster	State within Midwest Brazilian geographic region
44	SB1145	Cluster S1	352233236421225242223343	Orphan pattern	Orphan pattern	MS
45	SB1145	S1	3522332**42179*24222*38*	Orphan pattern	Orphan pattern	MS
49	SB0881	Cluster S2	352233234420225242223343	Cluster M1	Cluster A	MS
52	SB0881	S2	352233234420225242223343	M1	A	MS
10	SB0881	S2	352233236420225242223343	Orphan pattern	Orphan pattern	MS
35	SB0121	Cluster S3	352233255421235242223343	Cluster M2	Cluster B	MS
36	SB0121	S3	352233255421235242223343	M2	B	GO
11	SB0121	S3	35223325*421235242223343	Cluster M3	Cluster C	MT
22	SB0121	S3	352233256421235242223343	M3	C	GO
23	SB0121	S3	3522332**421235242223343	M3	C	MT
30	SB0121	S3	352233256421235242223323	Cluster M4	Cluster D	MT
37	SB0121	S3	35223325*421235242223323	M4	D	GO
33	SB0121	S3	352243255421235242223343	Orphan pattern	Orphan pattern	MT
39	SB0121	S3	352243256421235242223343	Orphan pattern	Orphan pattern	GO
48	SB0121	S3	352233256421235242221343	Orphan pattern	Orphan pattern	MS
17	SB0121	S3	352233254421235242223333	Orphan pattern	Orphan pattern	GO
05	SB0121	S3	352233235421235242223333	Orphan pattern	Orphan pattern	GO
04	SB0121	S3	351233236421235242223343	Orphan pattern	Orphan pattern	MS
38	SB0121	S3	352233234421135242223343	Orphan pattern	Orphan pattern	MS
15	SB0295	Cluster S4	352233236421235242223343	Orphan pattern	Orphan pattern	GO
16	SB0295	S4	352233236421235232223343	Orphan pattern	Orphan pattern	GO
13	SB0295	S4	342233256421235242223343	Orphan pattern	Orphan pattern	GO
12	SB0295	S4	352233256421234242222343	Orphan pattern	Orphan pattern	MT
25	SB0295	S4	35223426*421234242223343	Orphan pattern	Orphan pattern	MT
18	SB0295	S4	352233253421445242223343	Cluster M5	Orphan pattern	MT
09	SB1055	Orphan pattern	352233253421445242223343	M5	Orphan pattern	MS
01	SB1144	Cluster S5	352233234421135242221343	Orphan pattern	Orphan pattern	GO
21	SB1144	S5	3522332**421135242221383	Orphan pattern	Orphan pattern	GO
19	SB0140	Cluster S6	352223256421434242213353	Cluster M6	Cluster E	GO
20	SB0140	S6	352223256421434242213353	M6	E	GO
27	SB0140	S6	35222325*4214342422*3353	M6	E	MT
28	SB0140	S6	352223256421434242213353	M6	E	MT
46	SB0140	S6	352223245421434242223353	Orphan pattern	Orphan pattern	MS
29	SB0140	S6	342223254421534242222353	Orphan pattern	Orphan pattern	MS
14	SB0134	Orphan pattern	352234254421416242222342	Orphan pattern	Orphan pattern	GO
24	SB1136	Orphan pattern	352234054421216242222342	Orphan pattern	Orphan pattern	MT
03	SB1050	Orphan pattern	352233256220235221223323	Orphan pattern	Orphan pattern	GO

*or**failed to amplify; MT—Mato Grosso state; MS—Mato Grosso do Sul state; GO—Goiás state.