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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Anim Biotechnol. 2016 Oct;27(4):245–255. doi: 10.1080/10495398.2016.1178140

Figure 3.

Figure 3

Southern blot analysis of F0 transgenic boar TG7 and its F1 offspring’s genomic DNA. A. The location of probe and enzyme digestion sites for southern blot analysis in the transgene plasmid. pB 3′TRE, piggyBac transposon 3′ terminal repeat element. pB5′TREB, piggyBac transposon 5′ terminal repeat element. B. Southern blot analysis results. M=Marker; P(1C)=Positive control carrying 1 copy of EGFP gene, which was prepared by mixing 10μg of wild-type pig’s genomic DNA with 2.6 ×10−5 μg of a 9.0kb plasmid DNA fragment containing a EGFP gene; N=Negative control (Wild-type pig); TG=transgenic; WT=Wild-type; The F0 transgenic boar TG7 carries 4 copies (#1–#4) of EGFP transgene/piggyBac transposon.