Figure 4.

Identification of transposon (transgene) insertion sites in the genome of TG7 founder transgenic pig. A. Blast result and sequencing result of the first insertion site, which was at the non-coding intergenic sequences between nuclear receptor coactivator 1gene and collagen alpha-1 (I) chain-like gene on chromosome 3. B. Blast result and sequencing result of the second insertion site, which was at the non-coding intergenic sequences between two individual copies of beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform X2 gene on chromosome 3. C. Blast result and sequencing result of the third insertion site, which was at the noncoding 8^th intron of methymalonyl-CoA mutase gene on chromosome 7. D. Blast result and sequencing result of the fourth insertion site, which was at the non-coding intergenic sequences between signal sequence receptor alpha gene and cancer-associated gene 1 protein gene on chromosome 7. The PB 3′-TRE sites are flanked by the chromosomal sequences where the transposon inserted. The TTAA sequence on the border signifies transpositional integration of the PB transposon.