Table 3.
Porcine induced pluripotent stem cell lines used for nuclear transfer experiments
| Donor cells | In vitro development of embryos
|
|
No. of recipients | In utero development of fetuses
|
|||
|---|---|---|---|---|---|---|---|
| Fusion rate (%) | Cleavage rate (%) | Blastocyst rate (%) | No. of transferred embryos | No. of pregnant recipients (45d) | No. of cloned piglets | ||
| pM1–3-1, P5 | 147/210 (70.0) | 112/147 (76.2) | 9/147 (6.1) | 976 | 5 | 1 | 0 |
| pM1–4-2, P5 | 154/230 (67.0) | 90/154 (58.4) | 9/154 (5.8) | 1,012 | 5 | 0 | 0 |
| pM3-2-2, P5 | 175/227 (77.1) | 129/175 (73.7) | 16/175 (9.1) | 1,104 | 5 | 0 | 0 |
| pM3–4-1, P5 | 143/219 (65.3) | 98/143 (68.5) | 8/14 (5.6) | 991 | 5 | 0 | 0 |
| pM12-2-3, P5 | 161/208 (77.4) | 122/161 (75.8) | 18/161 (11.2) | 1,446 | 7 | 1 | 0 |
| pM12-6-2, P5 | 166/205 (81.0) | 134/166 (80.7) | 25/166 (15.1) | 1,505 | 7 | 2 | 0 |
| PFFs, P3-P5 | 156/184 (84.8) | 137/156 (87.8) | 48/156 (30.8) | 5,307 | 20 | 14 | 69 |
Abbreviation: PFFs, porcine fetal fibroblasts.