Skip to main content
. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biomaterials. 2016 Aug 17;106:167–179. doi: 10.1016/j.biomaterials.2016.08.024

Figure 10. Activation of signaling pathways and host blood vessel detection within in vivo grown CSG tooth bud constructs after 3 weeks.

Figure 10

IF analyses of SHH, RUNX2, BMP2 and CD31 in green and VM positive DM cells in red. The red staining identifies the biomimetic pulp organ layer where the DM cells are localized, while, the absence of red staining identifies the biomimetic enamel organ layer where the DE cells are localized. SHH was expressed throughout the CSG constructs in both biomimetic enamel and pulp organ layer (A, B). BMP2 was also expressed throughout the CSG constructs, but stronger BMP2 expression was observed in biomimetic pulp organ layers (C, D). RUNX2 was expressed in both biomimetic enamel and pulp organ layer (E, F), however, stronger RUNX2 expression was observed in the biomimetic epithelial organ layer (E, arrows). No CD31 positive staining was detected in the constructs (G, H). No positive staining was detected in the negative controls (I).