Figure 3. 3D GelMA-CS constructs cultured in osteogenic media for 24 h, 4, 7 and 12 days.
H&E images (A, B, C, D) revealed extracellular matrix formation and the morphology of the DE and DM cell sheets within the bilayer GelMA constructs. The arrows indicate the DE and the DM CSs. Pol images (E, F, G, H) show the organized collagen in the extracellular matrix. IF imaging (I, J, K, L) showed the expression of VM (green) by DM cells and CK18 (red) by the DE cells.