IHC analyses of dentin, enamel and bone specific markers. The odontoblast differentiation marker DSPP was highly expressed throughout the biomimetic pulp organ layer (A, B). Odontoblast/Osteoblast differentiation marker OC was expressed in the centers of the CSG constructs (C, D). Ameloblast differentiation marker AM was expressed throughout the CSG constructs in both biomimetic pulp and enamel organ layer (G, H), while AMBN was not detected in the CSG constructs (E, F). TEN was detected in the both biomimetic pulp and enamel organ layers of the CSG constructs (I, J).