Fig 2. High levels of autofluorescence and fast photobleaching of specific fluorescence when illuminating Qdots with metal halide.
FFPE section stained with Qdot 655 was imaged successively using mercury bulb, LED405 & Metal Halide source. The images are intensity images at 655nm. Red arrows: specific 655 fluorescence; green arrows: autofluorescence. The image taken with the first exposure to LED405nm illumination. (LED405 (1)) shows the specific staining with little autofluorescence when the section is illuminated with LED 405nm. Metal halide exposure led to high level autofluorescence and fast photobleaching of the specific fluorescence, confirmed with a second set of images with LED 405nm (LED405 (2)). Similar results were obtained with all other Qdots.