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. 2016 Sep 15;11(9):e0162419. doi: 10.1371/journal.pone.0162419

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© 2016 Prost et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — The graphs show the theoretical excitation spectra and emission spectra for the Qdot fluorophores used in the study—adapted from SemrockSearchlight.com. Note that at shorter wave lengths the excitation should be better. The excitation filter used is allowing wave lengths from 350 to 460 with a slightly better transmission rate around 405 than 425 (ASCII data in [15]); the dichroic mirror is 475nm and the emission filter is a 500nm long pass (Chroma filter set 32013). The illumination spectra for LED-405 & LED-425nm are shown in black.