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. 2016 Sep 15;11(9):e0162419. doi: 10.1371/journal.pone.0162419

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© 2016 Prost et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 9 — Comparison of unmixed specific fluorescence Cy3 (A) & Cy5 (B) when excited by white light LED (SOLA), PE2 (535nm & 615nm) or PE4000 (555 & 635nm). The same field of view illuminated with the indicated LED system was captured through the appropriate filter (for Cy3 & for Cy5) between 500 and 720nm. The fluorescence intensity (scaled counts/s) at emission peak wavelengths was obtained after spectral unmixing. The excitation peaks of Cy3 and Cy5 are 554 & 649 nm respectively. Despite the LAMs used with PE2 not being the optimum available, the results were better than with the white light system.