Table 5. Autofluorescence and specific fluorescence emission.

A
Autofluo at	525 nm	565 nm	585 nm	605 nm	625 nm	655 nm	705 nm
405	0.061 +/- 0.027	0.043 +/- 0.016	0.035 +/- 0.012	0.026 +/- 0.008	0.02 +/- 0.005	0.019 +/- 0.003	0.012 +/- 0.002
435	0.137 +/- 0.059	0.101 +/- 0.038	0.083 +/- 0.029	0.061 +/- 0.019	0.047 +/- 0.012	0.041 +/- 0.008	0.027 +/- 0.004
460	0.043 +/- 0.019	0.037 +/- 0.015	0.032 +/- 0.012	0.024 +/- 0.008	0.018 +/- 0.006	0.014 +/- 0.004	0.009 +/- 0.002
405+460	0.098 +/- 0.043	0.075 +/- 0.029	0.063 +/- 0.022	0.047 +/- 0.015	0.036 +/- 0.01	0.031 +/- 0.007	0.02 +/- 0.003
435+460	0.175 +/- 0.076	0.135 +/- 0.051	0.112 +/- 0.039	0.083 +/- 0.026	0.064 +/- 0.017	0.054 +/- 0.011	0.035 +/- 0.006
PE2	0.28 +/- 0.112	0.201 +/- 0.07	0.164 +/- 0.052	0.12 +/- 0.034	0.092 +/- 0.022	0.079 +/- 0.014	0.05 +/- 0.005
Sola 2	0.207 +/- 0.037	0.167 +/- 0.045	0.146 +/- 0.054	0.114 +/- 0.055	0.091 +/- 0.052	0.079 +/- 0.051	0.047 +/- 0.03
B
Specific fluo at	525 nm	565 nm	585 nm	605 nm	625 nm	655 nm	705 nm
405	0.282 +/- 0.093	0.917 +/- 0.269	0.898 +/- 0.191	0.183 +/- 0.034	0.988 +/- 0.362	0.542 +/- 0.125	0.238 +/- 0.044
435	0.536 +/- 0.171	1.686 +/- 0.492	1.68 +/- 0.354	0.335 +/- 0.068	1.926 +/- 0.715	0.789 +/- 0.195	0.351 +/- 0.067
460	0.205 +/- 0.069	0.584 +/- 0.172	0.589 +/- 0.123	0.147 +/- 0.033	0.757 +/- 0.284	0.302 +/- 0.075	0.154 +/- 0.03
405+460	0.49 +/- 0.162	1.459 +/- 0.432	1.467 +/- 0.312	0.282 +/- 0.055	1.676 +/- 0.625	0.63 +/- 0.167	0.298 +/- 0.061
435+460	0.753 +/- 0.241	2.2 +/- 0.65	2.221 +/- 0.474	0.447 +/- 0.09	2.575 +/- 0.96	0.905 +/- 0.249	0.392 +/- 0.082
PE2	1.033 +/- 0.299	3.137 +/- 0.711	2.287 +/- 0.603	0.51 +/- 0.096	3.402 +/- 1.054	1.319 +/- 0.335	0.594 +/- 0.094
Sola 2	0.839 +/- 0.312	2.659 +/- 0.846	2.184 +/- 0.69	0.521 +/- 0.126	3.153 +/- 1.282	1.168 +/- 0.302	0.888 +/- 0.146
C
ratio	525	565	585	605	625	655	705
405	4.60	21.51	25.79	7.10	48.24	29.22	19.62
435	3.91	16.63	20.18	5.48	40.92	19.36	12.92
460	4.74	15.64	18.32	6.12	42.12	21.46	18.02
405+460	5.02	19.52	23.43	6.05	46.31	20.25	14.93
435+460	4.31	16.28	19.77	5.38	40.45	16.83	11.09
PE2	3.69	15.63	13.95	4.27	37.07	16.78	11.89
Sola 2	4.06	15.97	14.93	4.57	34.67	14.78	18.80

A: intensity of autofluorescence at indicated wave lengths (average counts/s +/- sdv) as quantified in unstained kidney tubules (Fig 6A). red: worse levels of autofluorescence.

B: specific fluorescence emission at indicated wave lengths (average counts/s +/- sdv) as quantified in CD68 stained macrophages (Fig 6B). green: best levels of specific fluorescence.

C: ratio of specific emission over autofluorescence at indicated wave lengths.

The ratio gives the level of specific fluorescence at the peak of fluorescence of each Qdot.

Highlighted are the best ratios for each specific Qdot at peak of fluorescence. Note that the autoexposure time (with a long pass emission filter Chroma emitter E500lp) for Qdot605 is often high and close to that of the autofluorescence (Table 4) showing that the intensity of Q605 fluorescence in the stain is low, however, as the autofluorescence intensity around this wavelength is lower than in the green emission area (Fig 5) the specific fluorescence ratio is good (D) at this specific wave length.