Fig 1. Glucose and cAMP affects the type 3 fimbriae expression of K. pneumoniae CG43S3.

(A) K. pneumoniae CG43S3 was grown overnight at 37°C with agitation in LB broth supplemented with glucose and cAMP as indicated. K. pneumoniae CG43S3 WT, Δcrp, ΔcyaA, and ΔcpdA strains was grown overnight at 37°C with agitation in LB broth alone (B) or LB broth with 0.5% glucose (C) at 37°C with agitation to observe the type 3 fimbriae expression by Western blot analysis against MrkA (the upper panel) and GAPDH antiserum (the lower panel, for internal control). The MrkA and GAPDH proteins are indicated by an arrow, respectively.