Table 1. Analysis of CRP binding site in the upstream sequences of the c-di-GMP related ORFs in K. pneumoniae CG43 genome.
| Domain | Locus tag | Gene name | Typical CRP binding site TG(T/C)GA-N6-TC(A/G)CA | Positiona | Ratiob Δcrp/WT |
|---|---|---|---|---|---|
| GGDEF domain | |||||
| D364_04720 | - | TCAGA-TTGAAC-TCACA | -290 to -275 | 2.71±0.42 | |
| D364_06045 | - | TGCAA-GGAAAT-TCACG | -88 to -73 | 1.06±0.15 | |
| D364_09195 | - | TGCGC-GCAAAG-TCACC | -175 to -160 | 0.72±0.11 | |
| D364_15015 | GGTGA-GAATTG-TCCCA | -96 to -81 | 1.78±0.82 | ||
| EAL domain | |||||
| D364_06025 | - | TTTGA-TTTTTA-TTACA | -290 to -275 | 1.83±0.31 | |
| D364_08130 | - | AGTGG-AGGGAA-TCACA | -162 to -147 | 4.61±0.71 | |
| D364_13295 | - | TGCCA-CCTGGT-TCTCA | -112 to -97 | 2.45±0.23 | |
| D364_22720 | yjcC | TGTGA-ACTATA-TCACA | -382 to -367 | 2.85±0.24 | |
| GIL domain | |||||
| D364_19875 | bcsE | TGTCA-ATCAGG-GCGCA | -81 to -66 | 3.84±0.16 | |
aDistance to the translational start codon
bMean expression ratio of crp mutant relative to wild-type parental strain CG43S3