Fig 4. The evaluation of cytokines in the regulation of shrimp phagocytosis against the viral infection.

Shrimp were simultaneously injected with WSSV and PF4 (A), WSSV and Fas (B) or WSSV and IL-22 (C). PBS, PF4 alone, Fas alone, Il-22 alone and WSSV alone were included in the injections as controls. At 24 h after the injections, the shrimp hemocytes were subjected to the phagocytosis assay. The phagocytosis percentage was evaluated with flow cytometry. The experiments were biologically repeated three times. Statistically significant differences between treatments are shown with asterisks (**, p<0.01).